Transient light-activated gene expression in Chinese hamster ovary cells
نویسندگان
چکیده
منابع مشابه
Using transient transfected Chinese hamster ovary (CHO) cells by pET28a-LIC-NTF4 in gene therapy
Introduction: Neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. Also, neurotropic factors are growth factors like Neurotrophins that help neurons survive. Moreover, Neurotrophins differentiate between progenitor cells so that neurons are formed. Despite the fact that the majority of mammalian brain neurons are produced p...
متن کاملEXPRESSION OF HUMAN PROTEINASE 3 IN CHINESE HAMSTER OVARY CELLS (CHO-CELLS)
Proteinase 3(PR3) is a human polymorphonuclear leukocyte serine proteinase and is the main target antigen for antineutrophil cytoplasmic antibodies (ANCA) found in Wegener's granulomatosis (WG). We developed a stable expression system for conformationally intact recombinant PR3 (rPR3) in Chinese hamster ovary cells (CHO-cells). The part of PR3 cDNA that encoded the active form of PR3 was s...
متن کاملusing transient transfected chinese hamster ovary (cho) cells by pet28a-lic-ntf4 in gene therapy
introduction: neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. also, neurotropic factors are growth factors like neurotrophins that help neurons survive. moreover, neurotrophins differentiate between progenitor cells so that neurons are formed. despite the fact that the majority of mammalian brain neurons are produced p...
متن کاملCloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells
Background: The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. Methods: In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected ...
متن کاملEvaluation of transfection methods for transient gene expression in Chinese hamster ovary cells
Three transfection reagents, Lipofectamine 2000, TransIT-PRO and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced green fluorescent protein in Chinese hamster ovary cells. TransIT-PRO was found to be more efficient under the examined conditions, but comes at an increased cost compared to the widely used PEI.
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2021
ISSN: 1472-6750
DOI: 10.1186/s12896-021-00670-1